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The remaining homogenate was centrifuged at 40,000 g for 60 min, and the supernatant was used for the estimation of superoxide dismutase (SOD) and catalase (CAT)

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Levels of GFP-tagged stress proteins were monitored using a Nikon TiE2000 confocal laser microscope (lasers: 488 and 408 nm), equipped with a perfect focus system, a controlled temperature (37C) and CO 2 (5%) and an automated stage